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ultrascript 2 0 cdna synthesis kit  (PCR Biosystems Ltd)


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    Structured Review

    PCR Biosystems Ltd ultrascript 2 0 cdna synthesis kit
    Ultrascript 2 0 Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna/UltraScript+2%2E0+Reverse+Transcriptase/pmc13694090-75-12-17
    Average 94 stars, based on 92 article reviews
    ultrascript 2 0 cdna synthesis kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Coat Color‐Associated Transcriptomic Differences of Black and White Hortobágyi Racka Sheep Using the RNA‐Seq Technique
    Article Snippet: Primer Express v3.0.1 software (Applied Biosystems, Foster City, CA, USA) was used to design the primers and was validated using Primer Blast from NCBI (Ye et al. ) (Table ). .. The qPCRBIO cDNA Synthesis Kit (PCR Biosystems, London, UK) was used. ..

    Article Title: NQO1 acts as a context-dependent regulator of oxidative stress and biliary fibrosis in primary sclerosing cholangitis
    Article Snippet: The concentration and integrity of the extracted RNA were assessed via NanoDrop spectrophotometer (NanoDrop Technologies, DE, USA). .. Subsequently, 1 μg of total RNA was synthesized into cDNA using the UltraScript 2.0 cDNA Synthesis Kit (PCR BioSystems, London, UK). .. Quantitative PCR was conducted using the AccuPower 2× Greenstar qPCR Master Mix (Bioneer, Daejeon, Korea) on a StepOnePlus Real-Time PCR system (Applied Biosystems, CA, USA).

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Synthesized:

    Article Title: NQO1 acts as a context-dependent regulator of oxidative stress and biliary fibrosis in primary sclerosing cholangitis
    Article Snippet: The concentration and integrity of the extracted RNA were assessed via NanoDrop spectrophotometer (NanoDrop Technologies, DE, USA). .. Subsequently, 1 μg of total RNA was synthesized into cDNA using the UltraScript 2.0 cDNA Synthesis Kit (PCR BioSystems, London, UK). .. Quantitative PCR was conducted using the AccuPower 2× Greenstar qPCR Master Mix (Bioneer, Daejeon, Korea) on a StepOnePlus Real-Time PCR system (Applied Biosystems, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    SYBR Green Assay:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Amplification:

    Article Title: Generation of a recombinant, antibiotic resistance gene-free Leishmania tarentolae strain as a live vaccine platform
    Article Snippet: .. Approximately 2 μl of cDNA was mixed with 5 μM of each primer and qPCRBIO SyGreen mix High-ROX 2X (PCR BioSystems) for amplification. .. Reactions performed using StepOne Plus Real-time PCR system (Applied Biosystems by Thermo Fisher Scientific) under the following cycling conditions: 95 °C for 5 min, followed by 40 cycles of 95 °C for 10 s, 60 °C for 30 s. Data were analyzed using StepOne Software V2.3.

    Polymerase Chain Reaction:

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).



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